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Preventing cell misidentification in daily lab routine
Preventing cell misidentification in daily lab routines is essential for maintaining data integrity and ensuring accurate experimental outcomes. Implementing stringent protocols for cell handling, labeling, and documentation reduces the risk of misidentification errors. Regular verification of cell lines through authentication methods such as DNA profiling or short tandem repeat (STR) analysis is crucial to confirm cell identity. Maintaining meticulous records of cell passages, storage conditions, and experimental procedures facilitates traceability and accountability. Implementing standardized procedures for cell culture maintenance and authentication across the laboratory promotes consistency and reliability in research outcomes. Regular training and education of laboratory personnel on proper cell culture techniques and quality control measures reinforce best practices and minimize errors. Vigilance and attention to detail in every step of the experimental workflow are paramount to prevent cell misidentification and uphold scientific rigor.
About the Speaker
Dr. Charudutt Joshi
Medical Director, Genes India , ART Bank, Hyderabad
Dr Joshi has done his post graduation in life sciences, special training in ICSI IVF and sperm morphology from Belgium. He Has also done special training in laser assisted hatching from Belgium. Dr. Joshi has done special training for embryo freezing from KK women’s hospital, Singapore, has special training in PGD from Germany and a doctorate in life sciences. Dr. Joshi is presently working as lab director in Mohak Hi tech Laparoscopy and infertility centers in India and Asian countries. She is even a Executive committee member of MP ISAR Chapter, past vice president ACE(Academy of clinical embryologist and President ACE
Upcoming Case Discussions
Endometriosis and Infertility
Endometriosis is a condition where tissue similar to the lining of the uterus grows outside of it, often affecting the ovaries, fallopian tubes, and pelvic organs. This disorder can cause painful periods, pelvic pain, and, in many cases, infertility. The presence of endometrial-like tissue outside the uterus can disrupt normal reproductive function by causing inflammation, scarring, and adhesions, which can interfere with ovulation, fertilization, and embryo implantation. Approximately 30-50% of women with endometriosis experience infertility, making it one of the leading causes of reproductive challenges in affected individuals. However, treatment options like surgery, medication, or assisted reproductive technologies can help improve fertility outcomes.
Impact of Endometriosis on Fertility
Endometriosis, a condition where endometrial-like tissue grows outside the uterus, can significantly impact fertility. It causes inflammation, scarring, and adhesions that may distort pelvic anatomy, block fallopian tubes, and impair ovarian function. Endometriosis is also linked to hormonal imbalances and poor egg quality, reducing the chances of conception. Symptoms like chronic pelvic pain and painful intercourse further complicate fertility. Diagnosis often requires laparoscopy, while management includes pain relief, hormonal therapy, and assisted reproductive techniques like IVF. Early intervention with medical or surgical treatment can improve reproductive outcomes, but severe cases may necessitate advanced fertility treatments for conception.
Hyperlipidemia: From Diagnosis to Treatment
Hyperlipidemia is a condition characterized by elevated levels of lipids, such as cholesterol and triglycerides, in the blood, which can increase the risk of cardiovascular diseases. Diagnosis typically involves blood tests measuring lipid profiles, while treatment focuses on lifestyle changes, such as diet and exercise, alongside medications like statins to manage cholesterol levels and reduce cardiovascular risk. Regular monitoring is essential for effective management and prevention of complications.
Acne: Disorders and Treatment Approaches
Acne is a common dermatological condition caused by clogged pores, excess sebum production, bacterial growth, and inflammation. It can manifest as blackheads, whiteheads, papules, pustules, or cysts, often leading to scarring if untreated. Various factors, including hormonal changes, diet, stress, and genetics, influence its severity. Treatment approaches range from topical and oral medications, such as retinoids, antibiotics, and hormonal therapy, to advanced procedures like chemical peels and laser therapy. A personalized skincare regimen, along with lifestyle modifications, plays a crucial role in managing and preventing acne.
Abnormal Uterine Bleeding: Diagnosis and Management
Abnormal Uterine Bleeding (AUB) is a common gynecological condition characterized by bleeding outside the normal menstrual cycle, excessive flow, or irregular patterns. It can result from structural causes (polyps, fibroids, adenomyosis, malignancy) or non-structural factors (coagulopathy, ovulatory dysfunction, endometrial disorders). Diagnosis involves a thorough history, physical examination, laboratory tests, imaging (ultrasound), and sometimes hysteroscopy. Management depends on the underlying cause and includes hormonal therapy (oral contraceptives, progestins), non-hormonal options (NSAIDs, tranexamic acid), and surgical interventions (endometrial ablation, myomectomy, hysterectomy). A tailored approach ensures effective symptom control and improved quality of life. Early evaluation is key to preventing complications.
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