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Preventing cell misidentification in daily lab routine
Preventing cell misidentification in daily lab routines is essential for maintaining data integrity and ensuring accurate experimental outcomes. Implementing stringent protocols for cell handling, labeling, and documentation reduces the risk of misidentification errors. Regular verification of cell lines through authentication methods such as DNA profiling or short tandem repeat (STR) analysis is crucial to confirm cell identity. Maintaining meticulous records of cell passages, storage conditions, and experimental procedures facilitates traceability and accountability. Implementing standardized procedures for cell culture maintenance and authentication across the laboratory promotes consistency and reliability in research outcomes. Regular training and education of laboratory personnel on proper cell culture techniques and quality control measures reinforce best practices and minimize errors. Vigilance and attention to detail in every step of the experimental workflow are paramount to prevent cell misidentification and uphold scientific rigor.
About the Speaker
Dr. Charudutt Joshi
Medical Director Genes India , ART Bank, Hyderabad
Dr Joshi has done his post graduation in life sciences, special training in ICSI IVF and sperm morphology from Belgium. He Has also done special training in laser assisted hatching from Belgium. Dr. Joshi has done special training for embryo freezing from KK women’s hospital, Singapore, has special training in PGD from Germany and a doctorate in life sciences. Dr. Joshi is presently working as lab director in Mohak Hi tech Laparoscopy and infertility centers in India and Asian countries. She is even a Executive committee member of MP ISAR Chapter, past vice president ACE(Academy of clinical embryologist and President ACE
Upcoming Case Discussions
Ulcerative Colitis: Treatment and Management review
Respiratory diseases in children, such as asthma, pneumonia, and bronchiolitis, are significant causes of morbidity and mortality worldwide. These conditions often present with symptoms like wheezing, coughing, and difficulty breathing, requiring prompt diagnosis and management. Advances in medical care have improved the treatment outcomes, but disparities in healthcare access still pose challenges. Preventative measures, including vaccination and reducing exposure to environmental pollutants, play a crucial role in mitigating the impact of these diseases. Early intervention and comprehensive care are essential to enhance the quality of life for affected children.
Tobacco and Nicotine Control Laws
Tobacco and nicotine control laws aim to reduce the health risks associated with smoking and nicotine use. These regulations often include raising the legal purchasing age, restricting advertising, and imposing higher taxes on tobacco products. Many countries enforce plain packaging with health warnings to discourage use. Public smoking bans and restrictions on flavored nicotine products help protect non-smokers and reduce youth initiation. Additionally, emerging products like e-cigarettes are increasingly regulated under these laws to ensure public health safety
Approach to Sudden Sensorineural Hearing Loss
One typical complaint that gets referred to secondary care for an otolaryngologist's attention is hearing loss. Conductive and sensorineural hearing loss are the two categories of hearing loss. The majority of hearing loss is caused by sensorineural hearing loss (SNHL), which is the most prevalent form. Any cause of hearing loss resulting from a disorder of the central nervous system, auditory nerve, or cochlea is referred to as SNHL. An otolaryngologist, an audiologist, a radiologist, and a speech-language pathologist should be part of a multidisciplinary team that investigates and completes a comprehensive audiometric evaluation for patients with recent onset hearing loss.
Nutritional Strategies for Diabetes Management
Nutritional strategies play a vital role in managing diabetes by helping to regulate blood sugar levels and improve overall health. A balanced diet rich in whole grains, lean proteins, healthy fats, and fiber can promote better glycemic control. Portion control and carbohydrate counting are essential tools for managing post-meal blood sugar spikes. Regular intake of non-starchy vegetables and limiting processed foods and sugars are key components of a diabetes-friendly diet. Additionally, incorporating healthy snacks and maintaining consistent meal timing can help stabilize blood glucose levels and prevent complications.
Cryopreservation in ART
Cryopreservation in Assisted Reproductive Technology (ART) is a crucial technique used to freeze and store eggs, sperm, or embryos for future use. It allows individuals or couples to preserve fertility, especially before medical treatments like chemotherapy or for those delaying parenthood. The process involves rapid freezing to protect the cells from damage. Vitrification, a newer method, has significantly improved survival rates of frozen embryos and eggs. Cryopreservation also offers flexibility in ART cycles, improving the chances of successful pregnancies over time.
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Dr. Charudutt Joshi 's Talks on Assimilate
- 24th-May-2024, TIME : 5:00PM - 6:00PM
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Preventing cell misidentification in daily lab routines is essential for maintaining data integrity and ensuring accurate experimental outcomes. Implementing stringent protocols for cell handling, labeling, and documentation reduces the risk of misidentification errors. Regular verification of cell lines through authentication methods such as DNA profiling or short tandem repeat (STR) analysis is crucial to confirm cell identity. Maintaining meticulous records of cell passages, storage conditions, and experimental procedures facilitates traceability and accountability. Implementing standardized procedures for cell culture maintenance and authentication across the laboratory promotes consistency and reliability in research outcomes. Regular training and education of laboratory personnel on proper cell culture techniques and quality control measures reinforce best practices and minimize errors. Vigilance and attention to detail in every step of the experimental workflow are paramount to prevent cell misidentification and uphold scientific rigor.